The HTGC laboratory is subdivided into three separate rooms to allow for optimal workflow while maintaining the standards required by CAP/CLIA.
The sample accession/extraction room contains 2 workstations to scan the barcodes of each sample into the laboratory information management system (Clarity LIMS by Illumina). Within the Clarity LIMS each sample will be assigned to the appropriate workflow and therefore can be tracked electronically throughout the entire laboratory and analysis process. The sample management room also contains one Vantage robot by Hamilton and one Tecan Fluent 780 for liquid handling, two Chemagic 360 instruments by Perkin Elmer for 96-well or 24-well plate-based DNA and RNA isolation, one Synergy by BioTek and one Qubit by Invitrogen for quantification, and one Fragment Analyzer by Agilent for measuring RNA quality.
The library preparation and QC room contains two Hamilton Vantage robotics for automated library preparation, two Eppendorf epMotion 5075t NGS robotic handlers for automated library preparation and sample preparation for Mass Spec protocols, two Fragment Analyzers for library size and quality determination, one Tecan to measure library concentration, and one LightCycler by Roche for qPCR to measure library concentration as required, and two Chemical Fume Hoods.
The sequencing room contains two NovaSeq X Plus, one Novaseq 6000, and one MiSeqDX by Illumina for sequencing. This room also holds the Biomek FXP liquid handler by Beckman Coulter for the automated processing of 96-well format ThermoFisher microarray plates and the GeneTitan by ThermoFisher for integrated hybridization, staining and scanning of the PharmacoScan and other array Axiom varieties. It also contains one iScan System including a Tecan Freedom EVO 150 robotic handler, two Stellar Mass Spectrometers with Vanquish Neo UHPLC systems, and two Orbitrap Exploris 240 Mass Spectrometers with Vanquish Flex UHPLC systems.
The HTGC rooms and equipment are automatically monitored for temperature control and contain ample storage for samples and reagents in 4°C, -20°C, and -80°C units; biosafety cabinets and dead air hoods, and other small lab equipment required for optimized high-throughput processing.